Plasmid_Backbone
thrC

Part:BBa_K4074048

Designed by: MIT iGEM 2021   Group: iGEM21_MIT   (2021-10-01)


pSB_THRC

Contents: thrC locus
What is it?: A basic part - plasmid backbone
What does it do?: It codes for thrC homology arms, which allow for integration into the B. subtilis genome at the thrC locus.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1207
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 3313
    Illegal XbaI site found at 2205
    Illegal PstI site found at 2193
    Illegal PstI site found at 3527
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1207
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 3313
    Illegal PstI site found at 2193
    Illegal PstI site found at 3527
    Illegal NotI site found at 3520
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1207
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 3313
    Illegal BamHI site found at 2211
    Illegal XhoI site found at 171
    Illegal XhoI site found at 1063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1207
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 3313
    Illegal XbaI site found at 2205
    Illegal PstI site found at 2193
    Illegal PstI site found at 3527
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1207
    Illegal EcoRI site found at 2232
    Illegal EcoRI site found at 3313
    Illegal XbaI site found at 2205
    Illegal PstI site found at 2193
    Illegal PstI site found at 3527
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
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